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mouse antibody anti synaptobrevin 2  (Synaptic Systems)


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    Structured Review

    Synaptic Systems mouse antibody anti synaptobrevin 2
    Mouse Antibody Anti Synaptobrevin 2, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 96/100, based on 199 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti-synaptobrevin+2+(104+211)/104+211/pmc10175517-282-0-19
    Average 96 stars, based on 199 article reviews
    mouse antibody anti synaptobrevin 2 - by Bioz Stars, 2026-10
    96/100 stars

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    Incubation:

    Article Title: Experimental Parkinsonism induced by tetanus toxin injected into basal ganglia.
    Article Snippet: .. Subsequently, the sections were incubated with primary antibody (1:400 in blocking solution) specific for TeNT-cleaved VAMP/synaptobrevin raised in rabbit (produced by Ornella Rossetto, University of Padua, Italy) characterized by Fabris et al. (2022), or with mouse monoclonal antibody specific for full length TeNT or BoNT/B uncleaved VAMP-2 (Synaptic Systems, Cat#104211, RRID:AB_ 2619758) overnight at 4 C. After three washes in PBS, the sections were further incubated with fluorescently labelled secondary antibody (anti-rabbit IgG Fab2 Alexa Fluor 555 or anti-mouse IgG Fab2 Alexa Fluor 488, 1:400; CellSignaling Technology, Cat#4413, RRID:AB_ 10694110; Cat#4408, RRID:AB_10694704) for 2 h at room temperature in dark. ..

    Blocking Assay:

    Article Title: Experimental Parkinsonism induced by tetanus toxin injected into basal ganglia.
    Article Snippet: .. Subsequently, the sections were incubated with primary antibody (1:400 in blocking solution) specific for TeNT-cleaved VAMP/synaptobrevin raised in rabbit (produced by Ornella Rossetto, University of Padua, Italy) characterized by Fabris et al. (2022), or with mouse monoclonal antibody specific for full length TeNT or BoNT/B uncleaved VAMP-2 (Synaptic Systems, Cat#104211, RRID:AB_ 2619758) overnight at 4 C. After three washes in PBS, the sections were further incubated with fluorescently labelled secondary antibody (anti-rabbit IgG Fab2 Alexa Fluor 555 or anti-mouse IgG Fab2 Alexa Fluor 488, 1:400; CellSignaling Technology, Cat#4413, RRID:AB_ 10694110; Cat#4408, RRID:AB_10694704) for 2 h at room temperature in dark. ..

    Produced:

    Article Title: Experimental Parkinsonism induced by tetanus toxin injected into basal ganglia.
    Article Snippet: .. Subsequently, the sections were incubated with primary antibody (1:400 in blocking solution) specific for TeNT-cleaved VAMP/synaptobrevin raised in rabbit (produced by Ornella Rossetto, University of Padua, Italy) characterized by Fabris et al. (2022), or with mouse monoclonal antibody specific for full length TeNT or BoNT/B uncleaved VAMP-2 (Synaptic Systems, Cat#104211, RRID:AB_ 2619758) overnight at 4 C. After three washes in PBS, the sections were further incubated with fluorescently labelled secondary antibody (anti-rabbit IgG Fab2 Alexa Fluor 555 or anti-mouse IgG Fab2 Alexa Fluor 488, 1:400; CellSignaling Technology, Cat#4413, RRID:AB_ 10694110; Cat#4408, RRID:AB_10694704) for 2 h at room temperature in dark. ..

    Western Blot:

    Article Title: Presynaptic filopodia form kinapses and modulate membrane mechanics for synchronous neurotransmission and seizure generation
    Article Snippet: .. The following primary mouse antibodies were used in this study: synaptobrevin 2 (WB, 1:2000; Synaptic Systems, 104211, RRID:AB_2619757); synaptotagmin 1 ATTO647N (ICC, 1:200; Synaptic Systems, 105311AT1, RRID:AB_993036); GluA Oyster550 (ICC, 1:200; Synaptic Systems, 182411C3, RRID:AB_2619877); The following rabbit antibodies were used: synaptophysin 1 (WB, 1:1000; Synaptic Systems, 101 002, RRID:AB_1961591); SV2A (ICC, 1:1000; Abcam, ab32942, RRID:AB_778192); vGAT Sulfocyanine 3 (vGAT-Cy3) (ICC, 1:100; Synaptic Systems, 131103C3, RRID:AB_2189809); NHE1 (ICC, 1:200, Thermo Fisher Scientific, PA520444, RRID:AB_11153959). .. The following secondary antibodies were used in this study: donkey anti-rabbit Alexa Fluor 568 (ICC, 1:1000; Thermo Fisher Scientific, A10042, RRID:AB_2534017); goat anti-rabbit Alexa Fluor 488 (ICC, 1:1000; Thermo Fisher Scientific, A11008, RRID:AB_143165); goat anti-rabbit Alexa Fluor 647 (ICC, 1:1000; Thermo Fisher Scientific, A21244, RRID:AB_2535812); donkey anti-rabbit immunoglobulin G (IgG) IRDye 800 CW (WB, 1:10,000; LI-COR, 92532213, RRID:AB_2715510); goat anti-mouse IgG IRDye 800 CW (WB, 1:10,000; LI-COR, 92632210, RRID:AB_621842).

    Article Title: Synaptotagmin-11 Inhibits Synaptic Vesicle Endocytosis via Endophilin A1
    Article Snippet: .. The primary antibodies used for Western blotting were anti-synaptotagmin 11 (270003, Synaptic Systems), anti-clathrin heavy chain (610499, BD Transduction Laboratories), anti-AP2 α-adaptin (610501, BD Transduction Laboratories), anti-dynamin 1 (2441931, Millipore), anti-caveolin 1 (N-20) (sc-894, Santa Cruz Biotechnology), anti-synapsin 1 (106011, Synaptic Systems), anti-synaptotagmin 1 (105103, Synaptic Systems), anti-synaptophysin 1 (101011, Synaptic Systems), anti-synaptobrevin (104211, Synaptic Systems), anti-α-synuclein (610787, BD Transduction Laboratories), anti-β-actin (A1978, Sigma), anti–voltage-dependent anion channel (4866, Cell Signaling), anti-GAPDH (G8795, Sigma), anti-transferrin receptor (TfR, ab84036, Abcam), anti-TGN46 (ab16059, Abcam), anti-Na + /K + -ATPase α1 antibody (C464.6) (sc-21 712, Santa Cruz Biotechnology), anti-SNAP25 (111011, Synaptic Systems), anti-endophilin (D-3) (sc-48378, Santa Cruz Biotechnology), and anti-c-Myc (sc-789 and sc-40, Santa Cruz Biotechnology). .. The antibodies for immunofluorescence were anti-dynamin 1 (2441931, Millipore), anti-synapsin 1 (106011, Synaptic Systems), anti-synaptobrevin 2 (104211, Synaptic Systems), anti-c-Myc (sc-789 and sc-40, Santa Cruz Biotechnology; 2272S, CST), and anti-EndoA1 (65169S, CST).

    Article Title: α-Synuclein Facilitates Spontaneous Dopamine Release in a Calcium- and Phosphorylation-Dependent Manner
    Article Snippet: The membrane was first probed with anti-aSyn primary antibody (Cell Signalling Technology, D37A6, 1:1000) and HRP-conjugated anti-rabbit IgG secondary antibody (GE Healthcare, 1:4000). .. Blots were subsequently stripped and reprobed with anti-synaptobrevin (VAMP2) primary antibody (SYSY, 104211, 1:1000) followed by an HRP-conjugated anti-mouse IgG secondary antibody (GE Healthcare, 1:4000). (The raw Western blot images from 3 independent experiments are provided in Supplementary Fig 1) Densitometric analysis of the western blots was used to determine the percentage of aSyn present in vesicle-enriched fractions, normalised to VAMP2, which is abundantly found on synaptic vesicles ( ). .. Single-molecule localisation microscopy (direct STORM, dSTORM) was performed on a custom-built wide-field microscope based on an Olympus IX-73 frame (Olympus, Center Valley, PA).

    Immunocytochemistry:

    Article Title: Presynaptic filopodia form kinapses and modulate membrane mechanics for synchronous neurotransmission and seizure generation
    Article Snippet: .. The following primary mouse antibodies were used in this study: synaptobrevin 2 (WB, 1:2000; Synaptic Systems, 104211, RRID:AB_2619757); synaptotagmin 1 ATTO647N (ICC, 1:200; Synaptic Systems, 105311AT1, RRID:AB_993036); GluA Oyster550 (ICC, 1:200; Synaptic Systems, 182411C3, RRID:AB_2619877); The following rabbit antibodies were used: synaptophysin 1 (WB, 1:1000; Synaptic Systems, 101 002, RRID:AB_1961591); SV2A (ICC, 1:1000; Abcam, ab32942, RRID:AB_778192); vGAT Sulfocyanine 3 (vGAT-Cy3) (ICC, 1:100; Synaptic Systems, 131103C3, RRID:AB_2189809); NHE1 (ICC, 1:200, Thermo Fisher Scientific, PA520444, RRID:AB_11153959). .. The following secondary antibodies were used in this study: donkey anti-rabbit Alexa Fluor 568 (ICC, 1:1000; Thermo Fisher Scientific, A10042, RRID:AB_2534017); goat anti-rabbit Alexa Fluor 488 (ICC, 1:1000; Thermo Fisher Scientific, A11008, RRID:AB_143165); goat anti-rabbit Alexa Fluor 647 (ICC, 1:1000; Thermo Fisher Scientific, A21244, RRID:AB_2535812); donkey anti-rabbit immunoglobulin G (IgG) IRDye 800 CW (WB, 1:10,000; LI-COR, 92532213, RRID:AB_2715510); goat anti-mouse IgG IRDye 800 CW (WB, 1:10,000; LI-COR, 92632210, RRID:AB_621842).

    Control:

    Article Title: A belt-buckle checkpoint regulates the onset of botulinum neurotoxin intoxication.
    Article Snippet: .. Intact and cleaved SNAP-25 (AA1-197) were detected by the mouse monoclonal anti-SNAP-25 antibody clone 71.2 (#111111; Synaptic Systems GmbH, Göttingen, Germany), recognizing an epitope present in both entities, while VAMP-2, serving as a control protein, was detected by themousemonoclonal anti-VAMP-2 antibody clone 69.1 (#104211; Synaptic Systems GmbH, Göttingen, Germany). ..

    other:

    Article Title: Reduced synaptic proteins and SNARE complexes in Down syndrome with Alzheimer’s disease and the Dp16 mouse Down syndrome model: impact of APP gene dose
    Article Snippet: Mouse antibody anti-synaptobrevin 2 (1:5000; 104 211), rabbit anti-neurofilament L (171 002), and anti-syntaxin 1B (110 402) were purchased Synaptic Systems (Goettingen, Germany).



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